Experiment no. 05 Simultaneous Estimation Of Paracetamol And Ibuprofen By Uv Spectroscopy
Simultaneous Estimation Of Paracetamol And Ibuprofen By Uv Spectroscopy
S .R Yadav
Introduction Ibuprofen, 2-(4-isobutylphenyl)-propionic acid[15687-27-1], is non-steroidal anti-inflammatory drug that is available in a variety of preparations. It is commonly used in treatment of pain and inflammation in rheumatoid arthritis and other musculoskeletal disorders (adams et al., 1976). Paracetamol, acetaminophen[103-90-2], is a very widely used as analgesic and antipyretic drug. Its action is similar to aspirin, and is the most commonly used in pediatrics (hamm, 2000). Thus, tablets containing ibuprofen and paracetamol showed combined analgesic, anti-inflammatory and antipyretic action.
Criteria to apply simultaneous equation method
-Two absorbing species( x and y) ,each of which should have some absorbance at λ max of other
-The λ max two drugs should be reasonable different
-The two components should not interact chemically .
-They should obey beer-lambert law.
derivative of the ratio spectra ketotifen tablets
•A Shimadzu 1601 double beam UV–Vis spectrophotometer with a fixed slit width (2 nm) connected to an IBM compatible computer and a HP 600 inkjet printer was used.
•The bundled software was UV PC personal spectroscopy version 3.91 (Shimadzu).
•Materials and reagents
•Pharmaceutical grade of ibuprofen and paracetamol were kindly supplied by Rameda
• analytical grade methanol (Riedel-dehaen) was used throughout these experiments.
• The commercial Ketotifen tablets used containing 200 mg IB and 325 mg PA per tablet was manufactured by Sigma Company.
•Stock solutions of 1 mg/ml of IB and PA were prepared by dissolving 100 mg of each drug in 100 ml methanol.
•The standard solutions were prepared by dilution of the stock solutions with the same solvent to reach concentration range 2–20 and 1–15 μg/ml for IB and PA.
•Twenty tablets were weighed and finely powdered in a mortar, an amount of the tablets powder equivalent to 100 mg IB and 162.5 mg PA was dissolved in about 60 ml methanol and filtered in a 100 ml measuring flask, the residue was washed three times with 10 ml methanol, then completed to the mark with the same solvent, appropriate solutions were prepared by taking suitable aliquots of this solution.
method for IB-
•The UV absorption spectra of the solutions prepared at different concentrations (2–20 μg/ml) in its binary mixture with PA were recorded against methanol and, smoothed at Δλ = 2 nm then divided by the smoothed at Δλ = 2 nm spectrum of the standard solution of PA (20 μg/ml in methanol), the ratio spectra were obtained. The first derivative was calculated for the obtained spectra with Δλ = 4 nm. The amplitudes at 225.6 nm were measured and found to be proportional to the concentration of IB.
method for PA-
•The UV absorption spectra of the solutions prepared at different concentrations (1-15μg/ml) in its binary mixture with IB were recorded against methanol and smoothed at Δλ = 2 nm, then divided by the smoothed Δλ = 2 nm spectrum of standard solution of IB (14 μg/ml in methanol), the ratio spectra were obtained. The second derivative was calculated for the obtained spectra with Δλ = 8 nm. The amplitudes at 238.9 nm were measured and found to be proportional to the concentration of PA.
Result and discussion
Estimation of paracetamol and ibuprofen was achieved by simultaneous equation method by using uv spectrophotometer. The linearity was checked in different concentrations and beers law obeyed in the concentration range of 1- 15μg/ml and 2-20μg/ml for both paracetamol and ibuprofen.